Spring 2019

Document Type

Poster Session


Daniel Moore PhD


mosquito borne illness, CRISPR, Nix gene, doublesex


Female mosquitoes take blood from humans to gain protein needed to produce eggs, and in the process they can transmit viruses, such as Zika, Dengue, and chikungunya, or parasites, such as malaria. Male mosquitoes do not transmit diseases. This literature review will examine what is known about how sex is determined in mosquitoes and how this can be used to manipulate the females that spread diseases to humans. Using CRISPR Cas9, the lab of Zhijian Tu removed the Nix gene from mosquito embryos; all of these were feminized. Ectopic overexpression of Nix gene product in mosquito embryos caused even genetically female mosquitoes to have male genitalia. Nix has been shown to be a regulating factor for doublesex and fruitless, two other genes known to be involved in sex determination. The Nix gene has highly repetitive features similar to other genes in the Y chromosome of other organisms. Researchers in London have already demonstrated that CRISPR technology can be used to disrupt the female form of doublesex in a population of caged mosquitoes. The females become sterile and the population declines Nix could be used in a similar way to change female mosquitoes to harmless males.

Start Date

4-19-2019 9:00 AM



To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.