Study of the effects of CTHRC1, a protein involved in tissue repair, on the expression of energy metabolism enzymes

Presenter Information

Deepak Kandel, University of Southern MaineFollow

Document Type

Oral Presentation

Department

Biological Sciences

Faculty Mentor

Igor Prudovsky, PhD, Maine Medical Center Research Institute

Keywords

CTHRC1, Adipogenesis, Obesity, Protein, Immunoblotting, Enzymes

Abstract

CTHRC1 is a protein highly expressed in the tissues undergoing repair after damage. We have found that CTHRC1 suppresses adipogenesis and increases mitochondrial respiration. In the present study, we explored the effects of CTHRC1 on the expression of mitochondrial and glycolytic energy metabolism enzymes. 3T3L1 cells were incubate in the medium containing CTHRC1 or in control CTHRC1 free medium. The expression of metabolic enzymes was assessed using immunoblotting. We found that CTHRC1 significantly enhanced the expression of pyruvate kinase M2, an important glycolytic enzyme responsible for supply pyruvate, a major fuel of mitochondrial respiration.

Comments

Department of Biological Sciences at the University of Southern Maine, and Igor Prudovsky Lab at Maine Medical Center Research Institute

Open Access?

1