Date of Award

2016

Call Number

QR186.85 .K44 2016

Document Type

USM Access Thesis

Degree Name

Master of Science (MS)

Department

Applied Medical Science

First Advisor

Ah-Kau Ng, Ph.D

Second Advisor

S. Monroe Duboise,Ph.D

Third Advisor

Stephen C. Pelsue,Ph.D

Keywords

Cell-mediated immunity, murine single-domain antibodies

Abstract

This study represents a preliminary investigation of the murine singledomain antibody, 2C5.VH-CH1 isolated from a murine B cell-derived hybridoma 2C5 that secrets a monoclonal antibody of IgG1 isotype (designated as MAb 2C5) that binds the N-terminus of human osteopontin (N-OPN). OPN has become an area of intense interest in bone tissue development, cell-mediated immunity and in the survival and proliferation of cancer cells. It has also been implicated in several autoimmune disorders, including rheumatoid arthritis and multiple sclerosis. For these reasons, emphasis on OPN when researching cancer and autoimmune disorders is vital. Monoclonal antibodies and single-chain variable fragments have given rise to many effective molecular therapies, but issues regarding tumor infiltration, chemoresistance, chemotoxicity, and the blood-brain barrier continue to vex medical researchers.

Comments

This thesis is restricted to USM access only.

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